This antibody recognizes proteins with a molecular weight of 145kDa, identified as CD117 or p145kit. It is a rabbit polyclonal antibody that doesn’t interfere with the binding of the SCF to the c-kit. It also precipitates unoccupied and occupied forms of the c-kit. Binding of the SCF to the c-kit encoded receptor called tyrosine kinase will stimulate many biochemical responses that can culminate in cellular migration, survival, and proliferation. The C-kit can also be important for melanogenesis, gametogenesis, and hematopoiesis.
Information About It
The CD117 antibody is meant for research purposes and has no known clone. The immunogen is the synthetic peptide found in the cytoplasmic domain of the same protein. The isotype is the Rabbit IgG, and it has an undetermined epitope with a molecular weight of 145kDa.
IHC Procedures
The Immunohistochemistry method starts with using paraffin-embedded or Formalin-fixed tissues and deparaffinized slides. If you purchase the concentrated format of the product, you’ll need to dilute the antibody using a ratio of 1:100, though dilutions are estimates. You can also find a pre-diluted formulation, which may be suitable for your application.
To retrieve the antigen, you’ll want to boil the tissue section in a 10mM citrate buffer with a pH of 6.0 for 10 or 20 minutes, allowing it to cool to room temperature for an additional 20 minutes. Once cooled, you’ll need to incubate for 10 minutes at room temperature, as well. You should always wash the slides between steps with a PBS/0.05 percent Tween solution. You can detect the antibody as per the instruction in your visualization system. The positive control includes the skin, tonsil, and gastrointestinal stromal tumor, with cellular localization occurring in the cytoplasm.
The CD117 can be used for many purposes and research needs. Visit Spring BioScience today to learn more about purchase options.
