The CD4 antibody is a single chain transmembrane glycoprotein and is found on a T-cell subset that represents 45 percent of the peripheral blood lymphocytes. It is also found on 80 percent of thymocytes and on monocytes at a lower level. It can be used to recognize antigens presented by MHC class II by APCs and can serve as a receptor for HIV.
More Information
The CD4 antibody is intended to be used for research and shouldn’t be used for diagnostic testing. It has no known clone, and the immunogen is the synthetic peptide that corresponds to the internal region of the human CD4. The isotype is the Rabbit IgG, and it has no determined epitope. The molecular weight of this antibody is 59kDa, and it has been tested on humans.
Applications/Uses
These antibodies are designed for Immunohistochemistry applications only. You will need to use a paraffin-embedded or Formalin-fixed tissue specimen. Likewise, you will require deparaffinized slides and should choose graded alcohols, xylene, or a xylene alternative.
You’ll find a pre-diluted formulation, which means you will not have to dilute the product to get the results you seek. However, you can also find concentrated versions in a variety of sizes, which means you will need to dilute it using a ratio of one to 50. However, the dilution ratio is an estimate, so you should check protocols and methods beforehand.
To retrieve the antigen, you’ll need to boil tissue sections in an EDTA buffer with a pH of 6.0 for about 10 minutes, and then allow it to cool to room temperature. Incubation periods are 30 minutes at room temperature.
The CD4 antibody can be used for a variety of purposes. Visit Spring Bioscience to learn how to purchase it and find out more about it.
